Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_74 [Q5YLI5]  : 6-domain trypsin inhibitor (Fragment)

395Length

Nicotiana attenuataSpecie

-Gene Name

43755Mass

11Domain Count

Sequence :
     FASTA
VHRVSFLALL LLFGMSLLVS NVEHADAKAC PRNCDGRIAY EICPRSEEKK NDRICTNCCA GTKGCKYFSD 70
DGTFICEGES DPRNPKACPR NCDGRIAYGI CPRSEEKKND RICTNCCAGT KGCKYFSDDG TFICEGESDP 140
RNPKACPRNC DGRIAYGICP RSEEKKNDRI CTNCCAGTKG CKYFSDDGTF ICEGESDPRN PKACPRNCDG 210
RIAYEICPRS EEKKNDRICT NCCAGTKGCK YFSDDGTFIC EGESDPRNPK ACPRNCDGRI AYEICPRTEE 280
KKNDRICTNC CAGTKGCKYF SDDGTFVCEG ESDPRNPKAC PRNCDERIAY GICPRTEEKK NNQICTNCCA 350
GTKGCNYFSA NGTFICEGES EYVSKVDEYV HEVENDLQKS RVAVS 395
SIGNAL PEPTIDE
SEQUENCE Position
VHRVSFLALLLLFGMSLLVSNVEHADA
 1 ↔ 27
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-165
53 - 104 52
IRD-165
111 - 162 52
IRD-166
169 - 220 52
IRD-168
227 - 278 52
IRD-169
285 - 336 52
IRD-615
202 - 252 51
IRD-615
28 - 78 51
IRD-616
86 - 136 51
IRD-616
144 - 194 51
IRD-618
260 - 310 51
IRD-619
318 - 368 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.042
3.5 -21.4 -38.9
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q5YLI5 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AY297103
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 31 - 35
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 263 - 267
RL-1 CPRNC Trypsin 263 - 267
RL-1 CPRNC Trypsin 321 - 325
RL-1 CPRNC Trypsin 321 - 325
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-165 53 - 104 CPRNC 88 - 92 DPRNP 81 - 85 H-L Type (Type-I)
IRD-165 111 - 162 CPRNC 146 - 150 DPRNP 139 - 143 H-L Type (Type-I)
IRD-166 169 - 220 CPRNC 204 - 208 DPRNP 197 - 201 H-L Type (Type-I)
IRD-168 227 - 278 CPRNC 262 - 266 DPRNP 255 - 259 H-L Type (Type-I)
IRD-169 285 - 336 CPRNC 320 - 324 DPRNP 313 - 317 H-L Type (Type-I)
IRD-615 202 - 252 CPRNC 204 - 208 EEKKN 221 - 225 L-H Type (Type-II)
IRD-615 28 - 78 CPRNC 30 - 34 EEKKN 47 - 51 L-H Type (Type-II)
IRD-616 86 - 136 CPRNC 88 - 92 EEKKN 105 - 109 L-H Type (Type-II)
IRD-616 144 - 194 CPRNC 146 - 150 EEKKN 163 - 167 L-H Type (Type-II)
IRD-618 260 - 310 CPRNC 262 - 266 EEKKN 279 - 283 L-H Type (Type-II)
IRD-619 318 - 368 CPRNC 320 - 324 EEKKN 337 - 341 L-H Type (Type-II)
RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYEICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y E I C P R S


RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYEICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y E I C P R T


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDERIAYGICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D E R I A Y G I C P R T


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYEICPRTEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R T E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACPRNCDERIAYGICPRTEEKKNNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D E R I A Y G I C P R T E E K K N N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-165
 53 - 104 52
IRD-165
 111 - 162 52
IRD-166
 169 - 220 52
IRD-168
 227 - 278 52
IRD-169
 285 - 336 52
IRD-615
 202 - 252 51
IRD-615
 28 - 78 51
IRD-616
 86 - 136 51
IRD-616
 144 - 194 51
IRD-618
 260 - 310 51
IRD-619
 318 - 368 51
Amino Acid Count % by Frequency
Alanine 23 5.82
Cysteine 48 12.15
Aspartic acid 29 7.34
Glutamic acid 33 8.35
Phenylalanine 14 3.54
Glycine 33 8.35
Histidine 3 0.76
Isoleucine 23 5.82
Lysine 31 7.85
Leucine 8 2.03
Methionine 1 0.25
Asparagine 28 7.09
Proline 22 5.57
Glutamine 2 0.51
Arginine 30 7.59
Serine 22 5.57
Threonine 20 5.06
Valine 11 2.78
Tryptophan 0 0
Tyrosine 14 3.54