Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_8 [Q43710]  : Proteinase inhibitor type-2 TR8 (Proteinase inhibitor type II TR8)

223Length

Solanum lycopersicumSpecie

ARPIGene Name

24697Mass

5Domain Count

Sequence :
     FASTA
MAIYKVALLL LFGMILLASD FEHAKACTKE CDTRIDFGIC PLLETKRVEG LCTNCCAGKK GCKYFSKDGT 70
YICDGESEWV SEKNNNLKKA CTKECDTRID FGICPLLETK RVEGLCTNCC AGKKGCKYFS KDGTYICDGE 140
SEWVSEKDNN LEKDCTKECD TRIDFGICPL LETKRVKGLC TNCCAGKKGC KYFSADGTYI CDGESEWVSE 210
GENDLQKSNV AIS 223
SIGNAL PEPTIDE
SEQUENCE Position
MAIYKVALLLLFGMILLASDFEHA
 1 ↔ 24
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-18
50 - 107 58
IRD-19
114 - 171 58
IRD-375
25 - 75 51
IRD-375
89 - 139 51
IRD-376
153 - 203 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.037
-1.8 -14.4 -23.9
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q43710 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 L21194
L25128
REFERENCES
PubMed ID:     7903168    | 8159801    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-10 CTKEC Trypsin 28 - 32
RL-10 CTKEC Trypsin 92 - 96
RL-10 CTKEC Trypsin 92 - 96
RL-10 CTKEC Trypsin 156 - 160
RL-10 CTKEC Trypsin 156 - 160
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Roots Auxin 7903168
Roots Auxin 8159801
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
Trypsin 7903168
Trypsin 8159801
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-18 50 - 107 CTKEC 91 - 95 - H+L Type (Type-III)
IRD-19 114 - 171 CTKEC 155 - 159 EKDNN 146 - 150 H-L Type (Type-I)
IRD-375 25 - 75 CTKEC 27 - 31 ETKRV 44 - 48 L-H Type (Type-II)
IRD-375 89 - 139 CTKEC 91 - 95 ETKRV 108 - 112 L-H Type (Type-II)
IRD-376 153 - 203 CTKEC 155 - 159 ETKRV 172 - 176 L-H Type (Type-II)
GLCTNCCAGKKGCKYFSKDGTYICDGESEWVSEKNNNLKKACTKECDTRIDFGICPLL
CTKEC
H+L Type (Type-III)
3,46 ; 6,24 ; 7,42 ; 13,55
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58
G L C T N C C A G K K G C K Y F S K D G T Y I C D G E S E W V S E K N N N L K K A C T K E C D T R I D F G I C P L L


GLCTNCCAGKKGCKYFSKDGTYICDGESEWVSEKDNNLEKDCTKECDTRIDFGICPLL
CTKEC
EKDNN
H-L Type (Type-I)
3,46 ; 6,24 ; 7,42 ; 13,55
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58
G L C T N C C A G K K G C K Y F S K D G T Y I C D G E S E W V S E K D N N L E K D C T K E C D T R I D F G I C P L L


KACTKECDTRIDFGICPLLETKRVEGLCTNCCAGKKGCKYFSKDGTYICDG
CTKEC
ETKRV
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T K E C D T R I D F G I C P L L E T K R V E G L C T N C C A G K K G C K Y F S K D G T Y I C D G


KACTKECDTRIDFGICPLLETKRVEGLCTNCCAGKKGCKYFSKDGTYICDG
CTKEC
ETKRV
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T K E C D T R I D F G I C P L L E T K R V E G L C T N C C A G K K G C K Y F S K D G T Y I C D G


KDCTKECDTRIDFGICPLLETKRVKGLCTNCCAGKKGCKYFSADGTYICDG
CTKEC
ETKRV
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K D C T K E C D T R I D F G I C P L L E T K R V K G L C T N C C A G K K G C K Y F S A D G T Y I C D G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-18
 50 - 107 58
IRD-19
 114 - 171 58
IRD-375
 25 - 75 51
IRD-375
 89 - 139 51
IRD-376
 153 - 203 51
Amino Acid Count % by Frequency
Alanine 11 4.93
Cysteine 24 10.76
Aspartic acid 16 7.17
Glutamic acid 20 8.97
Phenylalanine 8 3.59
Glycine 20 8.97
Histidine 1 0.45
Isoleucine 12 5.38
Lysine 26 11.66
Leucine 18 8.07
Methionine 2 0.9
Asparagine 10 4.48
Proline 3 1.35
Glutamine 1 0.45
Arginine 6 2.69
Serine 12 5.38
Threonine 15 6.73
Valine 8 3.59
Tryptophan 3 1.35
Tyrosine 7 3.14