Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_9 [Q43502]  : Proteinase inhibitor type-2 CEVI57 (Proteinase inhibitor type II CEVI57)

201Length

Solanum lycopersicumSpecie

CEVI57Gene Name

21419Mass

5Domain Count

Sequence :
     FASTA
MAVYKVSFLA HLLVLGMYLL VSTVEHANAC TKECGNLGYG ICPGSEGSPE NPICTNCCSG YKGCNYYYAN 70
GTFICEGTSD PKNPNICPSY CDPQIAYSKC PRSEGKTIIY PTGCTTCCTG YKGCYYFGQD GEFVCEGESI 140
EPKGCTKECD PRVAYMTCPS SGLAKLNQVC VNCCSAGEGC KLYDNDGSLL CTGEPQSIST A 201
SIGNAL PEPTIDE
SEQUENCE Position
MAVYKVSFLAHLLVLGMYLLVST
 1 ↔ 23
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-20
112 - 161 50
IRD-276
52 - 98 47
IRD-418
143 - 193 51
IRD-471
28 - 77 50
IRD-505
85 - 137 53
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.47
-3.9 -16.1 -24.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q43502 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 X94946
REFERENCES
PubMed ID:     8672818    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-10 CTKEC Trypsin 31 - 35
RL-10 CTKEC Trypsin 146 - 150
RL-10 CTKEC Trypsin 146 - 150
RL-46 CPSYC Unknown 88 - 92
RL-46 CPSYC Unknown 88 - 92
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Roots viroid infection and ethephon treatment Gene structure 8672818
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-20 112 - 161 CTKEC 145 - 149 - H+L Type (Type-III)
IRD-276 52 - 98 CPSYC 87 - 91 DPKNP 80 - 84 H-L Type (Type-I)
IRD-418 143 - 193 CTKEC 145 - 149 GLAKL 162 - 166 L-H Type (Type-II)
IRD-471 28 - 77 CTKEC 30 - 34 EGSPE 46 - 50 L-H Type (Type-II)
IRD-505 85 - 137 CPSYC 87 - 91 EGKTI 104 - 108 L-H Type (Type-II)
TGCTTCCTGYKGCYYFGQDGEFVCEGESIEPKGCTKECDPRVAYMTCPSS
CTKEC
H+L Type (Type-III)
3,38 ; 6,24 ; 7,34 ; 13,47
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
T G C T T C C T G Y K G C Y Y F G Q D G E F V C E G E S I E P K G C T K E C D P R V A Y M T C P S S


PICTNCCSGYKGCNYYYANGTFICEGTSDPKNPNICPSYCDPQIAYS
CPSYC
DPKNP
H-L Type (Type-I)
6,24 ; 7,13 ; 36,40
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47
P I C T N C C S G Y K G C N Y Y Y A N G T F I C E G T S D P K N P N I C P S Y C D P Q I A Y S


KGCTKECDPRVAYMTCPSSGLAKLNQVCVNCCSAGEGCKLYDNDGSLLCTG
CTKEC
GLAKL
L-H Type (Type-II)
3,31 ; 7,32 ; 16,28 ; 38,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C T K E C D P R V A Y M T C P S S G L A K L N Q V C V N C C S A G E G C K L Y D N D G S L L C T G


NACTKECGNLGYGICPGSEGSPENPICTNCCSGYKGCNYYYANGTFICEG
CTKEC
EGSPE
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
N A C T K E C G N L G Y G I C P G S E G S P E N P I C T N C C S G Y K G C N Y Y Y A N G T F I C E G


NICPSYCDPQIAYSKCPRSEGKTIIYPTGCTTCCTGYKGCYYFGQDGEFVCEG
CPSYC
EGKTI
L-H Type (Type-II)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
N I C P S Y C D P Q I A Y S K C P R S E G K T I I Y P T G C T T C C T G Y K G C Y Y F G Q D G E F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-20
 112 - 161 50
IRD-276
 52 - 98 47
IRD-418
 143 - 193 51
IRD-471
 28 - 77 50
IRD-505
 85 - 137 53
Amino Acid Count % by Frequency
Alanine 10 4.98
Cysteine 24 11.94
Aspartic acid 6 2.99
Glutamic acid 13 6.47
Phenylalanine 4 1.99
Glycine 23 11.44
Histidine 2 1
Isoleucine 9 4.48
Lysine 11 5.47
Leucine 12 5.97
Methionine 3 1.49
Asparagine 11 5.47
Proline 13 6.47
Glutamine 4 1.99
Arginine 2 1
Serine 16 7.96
Threonine 14 6.97
Valine 9 4.48
Tryptophan 0 0
Tyrosine 15 7.46